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Sub-Cellular Nanosurgery in Live Cells
Using Ultrashort Laser Pulses

A femtosecond laser is used to perform “nano” surgery on a living cell. The actin filaments in the cell have been fluorescently labeled using green fluorescent protein (GFP). When a single filament is cut, it retracts, much like a violin string that has broken. This demonstrates that the actin filaments in the cell are under tension, and hence are being “pulled” apart. As a result, the filaments retract when they are cut, as shown in the figure. This provides critical insight into the behavior of cytoskeletal network that plays a key role in determining the mechanical properties of the cell.


Last Modified October 19, 2006.

  

Last Modified May 19, 2008.  Graphics by Experimental Soft Condensed Matter Group.   Website by Carole Hoppe Mezian.