Sub-Cellular Nanosurgery in Live Cells Using Ultrashort Laser Pulses
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A femtosecond laser is used to perform “nano” surgery on
a living cell. The actin filaments in the cell have been fluorescently
labeled using green fluorescent protein (GFP). When a single filament
is cut, it retracts, much like a violin string that has broken. This
demonstrates that the actin filaments in the cell are under tension,
and hence are being “pulled” apart. As a result, the filaments
retract when they are cut, as shown in the figure. This provides critical
insight into the behavior of cytoskeletal network that plays a key role
in determining the mechanical properties of the cell.
Last Modified October 19, 2006.
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